The other company I’ve are almost clear and the agar crumbles apart when making transfers. Follow this and you'll get great plates - with no lumps, bubbles or excess moisture - every time. The agar plate is incubated for 18-24 hours (or longer if necessary), at a temperature suitable for the test microorganism. Allow the reagent to absorb into the plate. In the above picture of a mixed culture, an agar plate that has been exposed to the air and many different colony morphologies can be identified. If antimicrobial agent leaches from the object into the agar and then exerts a growth-inhibiting effect, then a clear zone (the zone of inhibition) appears around the test product. Sometime I see colony like dots on plates sometime clear but dried agar. Making agar plates, whether they contain LB, M9 or any other medium, is a simple procedure. In DNase agar without indicators: Flood the surface of agar with 1N HCL solution. After incubation observe the color change in DNase with methyl green. Neisseria meningitidis: Growth on chocolate agar is greyish, non-hemolytic, round, convex, smooth, moist, glistening colonies with a clearly defined edge. Whenever I use LB agar plates with either kanamycin or amp my plates get dried overnight. Tip off the excess acid. Expected Results Nine obviously different colonies are numbered: some colony types recur in various areas of the plate (note # 3 and # 4). https://www.the-odin.com/making-agar-plates-for-bacterial-growth Myc made my life so much easier! But there are a few finer points that will kill your experiment, make a mess or just cause you inconvenience if you get them wrong. Incubate the plate at 35-37°C for 24 hours. Observe for clear zone around the colonies within 5 minutes. So let's put on the record exactly how to make the perfect agar plate. Tweezers of pipettes are useful for transferring the seed or tissue to the prepared agar. Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10 mL of agar and on which you can individually distinguish a maximum of ~100 colonies *Pro-Tip* If you will be screening a large number of colonies, we recommend using larger plates. I ordered plates from another company once before and the difference in quality was night and day. I usually prepare agar and store it. Just looking at mycs plates you can tell they are great quality the agar has a rich golden color and the viscosity is great ! Antibiotics or antiseptics can be tested experimentally on pre-prepared agar plates. Germination varies by species but is generally twice as fast as other germination methods. Prepoured, 100x15 mm plates with Luria-Bertani (LB) Agar pH: 7.0 Keep away from continuous exposure to light Contains: 1% tryptone 0.5% yeast extract 1.0% NaCl 1.5% agar All plates have a guaranteed shelf life of 5 weeks from date of shipment. 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